The aim of this book is to provide detailed protocols for studying the molecular biology of the pathogen Mycobacterium tuberculosis, and its int- actions with host cells. As established mycobacterial laboratories move - wards exploiting the genome, and laboratories with expertise in other fields apply them to mycobacteria, both traditional and novel methodologies need to be reviewed. Thus the chapters in Mycobacterium tuberculosis Protocols range from perspectives on storage of strains and safety issues to the application of the latest functional genomics technologies. The last few years have been remarkable ones for research into M. tuber- losis. The most important landmark by far has been the completion of the genome sequence of the widely studied H37Rv strain (1). We can now predict every protein and RNA molecule made by the pathogen. This information is or will soon be enriched by the addition of genome sequences of other strains from the M. tuberculosis complex: a second strain of M. tuberculosis, My- bacterium bovis, and the vaccine strain, M. bovis BCG. Valuable comparative data will also be provided by the genome sequences of Mycobacterium leprae, Mycobacterium avium, and Streptomyces coelicolor. Another recent milestone for M. tuberculosis has been the development of efficient mutagenesis me- odologies, the lack of which has been a major handicap in functional studies.

Confusion about governance abounds. We lack appreciation of how different traditions of thought in the social sciences contribute to our understanding. Governance Theory and Practice tackles these issues head on, using a cross-disciplinary approach from political science, public administration, international relations, development studies, sociology and economics. Looking at collective decision making in societal systems or organisations, the book provides guidance on how power should be distributed, who should be involved in a decision, and how policy should be enforced.
The growing interest in governance is precisely because established institutional forms of governance appear under challenge and new forms appear to be emerging. This reality is compelling new ways of thinking about collective decision-making at a range of levels. This book explores three critical areas of practice: environmental, corporate and participatory governance. Knowledge of these extensive intellectual resources will enrich academic debate and enable those interested in reforming governance to move towards a wider vision.

Re-energizing Citizenship examines the dual character of civil society. The book provides a critical examination of attempts to re-energize citizenship in a range of contexts and offers insights into what works.

"Re-energizing Citizenship" examines the dual character of civil society. The malaise in civil society is seen as a key to understanding anti-social behaviour, the weaknesses of regeneration schemes and the divisions and antagonisms of our societies. Yet the resources and skills hidden away in civil society, if they could be unlocked, are seen as the key to effective intervention. The book provides a critical examination of attempts to re-energize citizenship in a range of contexts and offers insights into what works.

The aim of this book is to provide detailed protocols for studying the molecular biology of the pathogen Mycobacterium tuberculosis, and its int- actions with host cells. As established mycobacterial laboratories move - wards exploiting the genome, and laboratories with expertise in other fields apply them to mycobacteria, both traditional and novel methodologies need to be reviewed. Thus the chapters in Mycobacterium tuberculosis Protocols range from perspectives on storage of strains and safety issues to the application of the latest functional genomics technologies. The last few years have been remarkable ones for research into M. tuber- losis. The most important landmark by far has been the completion of the genome sequence of the widely studied H37Rv strain (1). We can now predict every protein and RNA molecule made by the pathogen. This information is or will soon be enriched by the addition of genome sequences of other strains from the M. tuberculosis complex: a second strain of M. tuberculosis, My- bacterium bovis, and the vaccine strain, M. bovis BCG. Valuable comparative data will also be provided by the genome sequences of Mycobacterium leprae, Mycobacterium avium, and Streptomyces coelicolor. Another recent milestone for M. tuberculosis has been the development of efficient mutagenesis me- odologies, the lack of which has been a major handicap in functional studies.

From 1979 to 1997 Britain was a laboratory for experiments in local governance as the control and delivery of local services was switched from elected councils to appointed boards (guangos), private companies or self-management. This book is about four models of local governance: the traditional "localist" model, the New Right's "individualist" model, the New Left's "mobilization" model, and government's own "centralist" model. It tests them against public opinion as expressed in 2203 interviews with ordinary citizens, 788 with councilors, and 903 with members of appointed boards.

From 1979 to 1997 Britain was a laboratory for experiments in local governance as the control and delivery of local services was switched from elected councils to appointed boards (quangos), private companies or self-management. This book is about four models of local governance: the traditional 'localist' model, the New Right's 'individual' model, the New Left's 'mobilisation' model, and government's own 'centralist' model. It tests them against public opinion as expressed in 2203 interviews with ordinary citizens, 788 with councillors, and 902 with members of appointed boards.

Confusion about governance abounds. Many lack appreciation of how different traditions of thought in the social sciences contribute to our understanding. This book tackles these weaknesses head on and aims to provide a wider vision of the area, examining three critical areas of practice: environmental, corporate and participatory governance.